STING Induces Liver Ischemia-Reperfusion Injury by Promoting Calcium-Dependent Caspase 1-GSDMD Processing in Macrophages

Figure 4

Knockdown of STING in liver macrophages reduces caspase 1-GSDMD expression and H/R-induced inflammation. KCs were isolated from the liver and pretreated with STING-specific siRNA (20 μM) or nonspecific siRNA (scramble) before H/R modeling. (a) The levels of STING, caspase 1, cleaved-caspase 1, GSDMD, and GSDMD-N in each group were measured by Western blotting. (b) The mRNA levels of STING were measured by quantitative RT-PCR. (c)–(g) Relative expression of STING, caspase 1, cleaved-caspase 1, GSDMD, and GSDMD-N in each group. (h) Caspase 1 activity was measured with a caspase 1 assay kit. (i) Supernatant LDH levels were measured. (j) The colocalization of caspase 1 and STING in KCs was measured by confocal laser scanning microscopy in each group (scale bar, 50 μm). (k, l) The levels of cytokines (IL-1β and IL-18) in the cell culture supernatant were measured by ELISA. All data are shown as the , (). , , and .

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