STING Induces Liver Ischemia-Reperfusion Injury by Promoting Calcium-Dependent Caspase 1-GSDMD Processing in Macrophages

Figure 5

Inhibition of caspase 1 attenuates liver IRI mediated by GSDMD in macrophages. VX-765 (50 mg/kg) was administered intraperitoneally 1 h before liver ischemia. C57/B6 mice were subjected to liver IRI modeling, and KCs were isolated from the liver in each group. (a) The levels of caspase 1, cleaved-caspase 1, GSDMD, and GSDMD-N in each group were measured by Western blotting. (b)–(e) Relative expression of caspase 1, cleaved-caspase 1, GSDMD, and GSDMD-N in each group. (f) Caspase 1 activity was measured with a caspase 1 assay kit. (g)–(i) Serum levels of ALT, AST, and LDH were measured. (j) The levels of cytokines (IL-1β and IL-18) in the cell culture supernatant were measured by ELISA. (k, l) H&E-stained sections of livers; average Suzuki scores were based on H&E-stained liver sections from different groups of mice (scale bar, 100 μm). (m) Transmission electron microscopy (TEM) was used to observe the ultrastructural changes in KCs (original magnification, ×20000). The red arrow indicates the incomplete structure of an organelle, and the blue arrow indicates discontinuity in the cell membrane. All data are shown as the ( mice per group). , , and .

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