The lncRNA-AK046375 Upregulates Metallothionein-2 by Sequestering miR-491-5p to Relieve the Brain Oxidative Stress Burden after Traumatic Brain Injury

Figure 1

AK046375 significantly upregulates the expression of MT2 in primary cortical neurons and astrocytes. (1) Quantitative PCR analysis of AK046375 levels in mouse primary cortical neurons (a) and astrocytes (e) after transfection with either overexpression or knockdown AK046375 adenovirus or their responding control vector viruses. (2) Quantitative PCR analysis of MT2-mRNA levels and western blotting results of MT2 in mouse primary cortical neurons (b, c) and astrocytes (f, g) after transfection with either overexpression or knockdown AK046375 adenovirus or their corresponding vector viruses. (3) Immunofluorescence images of MT2 in primary neurons (d) and astrocytes (h) with different AK046375 levels and their quantification. β3-Tubulin is a neuronal marker, and glial fibrillary acidic protein (GFAP) is an astrocytic marker (/group, , vs. the overexpression vector group, ^& vs. the knockdown vector group by one-way ANOVA;  μm, 400x).

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