[Retracted] Exosomal miR-27b-3p Derived from Hypoxic Cardiac Microvascular Endothelial Cells Alleviates Rat Myocardial Ischemia/Reperfusion Injury through Inhibiting Oxidative Stress-Induced Pyroptosis via Foxo1/GSDMD Signaling

Figure 2

CMEC-derived exosomes prevent I/R injury and pyroptosis in the rat heart. Exosomes (EXO) were injected into the rat heart (intramyocardial injection). (a) TTC staining was used for the indication of the infarcted size in the rat heart. (b) The expression of Foxo1 protein and pyroptosis-related proteins in heart of rat with different treatments (). (c) The production of serum creatine kinase (CK), lactate dehydrogenase (LDH), interleukin- (IL-) 1β, and IL-18 by ELISA assay (). (d) The relative expression of miR-27b-3p, miR-17-1-2p, and miR-217-3p in heart of rat with different treatments (). (e) The relative expression of miR-27b-3p, miR-17-1-2p, and miR-217-3p in exosomes from CMECs cultured under normal and hypoxic conditions, respectively (). The sham group (), without ligation and reperfusion; the I/R group (), ischemia-reperfusion injury. The I/R+normal-EXO group (), exosomes, rats were administrated with exosomes (10 μl, particles; intramyocardial injection) derived from CMECs cultured under normal conditions; the I/R+hypoxia-EXO group (), rats were administrated with exosomes (10 μl, particles; intramyocardial injection) derived from CMECs cultured under hypoxic conditions.

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