[Retracted] Exosomal miR-27b-3p Derived from Hypoxic Cardiac Microvascular Endothelial Cells Alleviates Rat Myocardial Ischemia/Reperfusion Injury through Inhibiting Oxidative Stress-Induced Pyroptosis via Foxo1/GSDMD Signaling

Figure 3

miR-27b-3p is necessary for the cytoprotection of exosomes derived from CMECs in H9C2 cells. (a) The relative expression level of miR-27b-3p in patients and normal people (). (b) The relative expression level of miR-27b-3p in exosomes derived from CMECs cultured under hypoxic conditions with and without miRNA inhibitors (). (c) The relative expression level of miR-27b-3p in H9C2 cells with different treatments (). (d) Fluorescence analysis for the effect of the uptake of PKH67-labeled exosomes to the cytoskeleton of H9C2 cells.  μm. (e) ELISA assay of the lactate dehydrogenase (LDH), interleukin- (IL-) 1β, and IL-18 in H9C2 cell culture medium (). (f) The expression of Foxo1 protein and pyroptosis-related proteins in H9C2 cells with different treatments. (g) Result of cell viability using CCK8 assay (). (h), the expression of ROS and SOD in H9C2 cells after different treatments. H/R: hypoxia/reoxygenation condition (2 h hypoxia: 5% CO₂, and 95% N₂; and 4 h normal conditions: 95% air plus 5% CO₂). EXO: exosomes. NC-EXO or inhibitor-EXO indicated exosomes were derived from CMECs with pretreatment of negative control (NC) or miR-27b-3p inhibitor in hypoxic conditions.

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