[Retracted] Exosomal miR-27b-3p Derived from Hypoxic Cardiac Microvascular Endothelial Cells Alleviates Rat Myocardial Ischemia/Reperfusion Injury through Inhibiting Oxidative Stress-Induced Pyroptosis via Foxo1/GSDMD Signaling

Figure 5

miR-27b-3p inhibition and Foxo1 expression show synergistically promotes I/R injury in rat heart. (a) The relative expression level of miR-27b-3p in peri-infarct heart tissues after I/R injury (). (b) The images and statistical results of infarcted sizes in rat heart (after the I/R model was established, lentiviral plasmid expressing Foxo 1 and exosomes (EXO) were injected into rat heart) (). (c) The images and statistical results of infarcted sizes in the rat heart (lentiviral plasmid expressing Foxo 1 and exosomes (EXO) were injected into rat heart; then, I/R model was established) (). (d) The representative images of histology (HE staining) of rat heart tissues (magnification, ×200, ×400; scale bar: 100, 50 μm, respectively). (e) Western blot analysis of Foxo1 and pyroptosis-related proteins in the rat heart in response to different treatments. (f) The representative images of Foxo1 immunochemistry (magnification, ×200 and ×400; and 50 μm, respectively). (g, h) The serum level of creatine kinase (CK), lactate dehydrogenase (LDH), interleukin- (IL-) 1β, and IL-18 in the rat heart ().

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