[Retracted] Exosomal miR-27b-3p Derived from Hypoxic Cardiac Microvascular Endothelial Cells Alleviates Rat Myocardial Ischemia/Reperfusion Injury through Inhibiting Oxidative Stress-Induced Pyroptosis via Foxo1/GSDMD Signaling

Figure 6

miR-27b-3p controls the methylation of GSDMD promoter and Foxo1 promotes histone acetylation of GSDMD. (a) H9C2 cells were transfected with mi-27b-3p mimics or inhibiter; the methylation status of GSDMD promoter were detected by MSP. (b) The two predicted binding sites of Foxo1 in the promoter of the GSDMD gene. Their binding activity is predicted in JASPAR. (c) The Dual-Luciferase Reporter assay confirmed the interaction between Foxo1 and GSDMD at the two sites (). (d) The inhibition of miR-27b-3p in exosome derived from hypoxic CMECs increased the enrichment of GSDMD DNA (). (e, f) The ChiP-PCR results of GSDMD in 293 cells treated with Foxo1 expressing plasmids ().

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