Glycyrrhizin Attenuates Hypoxic-Ischemic Brain Damage by Inhibiting Ferroptosis and Neuroinflammation in Neonatal Rats via the HMGB1/GPX4 Pathway

Figure 2

Glycyrrhizin inhibits HI-induced neuronal ferroptosis. (a)–(c) Western blots and average data for HMGB1 and GPX4 in the injured cortex at 72 h after HI (). (d) Representative immunohistochemical images of the GPX4 expression in the cerebral cortex. The arrows indicate GPX4+ cells. (e)–(i) Ferroptosis-related genes, including ATP5G3, CS, PTGS2, IREB2, and RPL8, were analyzed by qPCR (). (j) Western blots and the average data for GPX4 in vitro (). , vs. the Con group; # vs. the HI group or OGD group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)