Nerve Growth Factor (NGF) Encourages the Neuroinvasive Potential of Pancreatic Cancer Cells by Activating the Warburg Effect and Promoting Tumor Derived Exosomal miRNA-21 Expression

<div>miR-21-5p inhibitor suppressed PNI in PC cells to relieve nociceptive transmission. MiaPaCa-2 cells transfected with miR-21-5p inhibitor were cocultured with DRG cells for 48 h. (a) Diagrammatic sketch of MiaPaCa-2 and DGR cell coculture. (b) RT-qPCR was employed to test miR-21-5p level in DGR cells. (c-d) Transwell was used to detect migration of DGR cells. (e-g) Glucose uptake, lactate production, and ATP assays were employed to test glucose uptake, lactate production, and ATP accumulation. (h) Extracellular acidification rate (ECAR) was employed to test and detect the glycolytic capacity of DGR cells. 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Oxidative Medicine and Cellular Longevity

fig6

Figure 6

Figure 6: Nerve Growth Factor (NGF) Encourages the Neuroinvasive Potential of Pancreatic Cancer Cells by Activating the Warburg Effect and Promoting Tumor Derived Exosomal miRNA-21 Expression 

Figure 6 | Nerve Growth Factor (NGF) Encourages the Neuroinvasive Potential of Pancreatic Cancer Cells by Activating the Warburg Effect and Promoting Tumor Derived Exosomal miRNA-21 Expression 