Nerve Growth Factor (NGF) Encourages the Neuroinvasive Potential of Pancreatic Cancer Cells by Activating the Warburg Effect and Promoting Tumor Derived Exosomal miRNA-21 Expression

Figure 7

NGF encouraged PC cell neuroinvasive potential by activating the Warburg effect and upregulating TDE-miR-21-5p. Rescue experiments were performed by using cotreatment of 100 ng/ml of Tanezumab and miR-21-5p mimic on MiaPaCa-2 cells for 48 h, followed by coculture with DRG cells. (a) RT-qPCR was employed to test miR-21-5p expression in MiaPaCa-2 and DGR cells cotreatment of Tanezumab and miR-21-5p mimic. (b-c) Neuroinvasion index and DRG growth index are illustrated. (d-g) Transwell was used to detect invasion and migration of MiaPaCa-2 and DGR cells cotreatment of Tanezumab and miR-21-5p mimic. (h-i) Western blotting was employed to test TRPV1 expression in DGR cells. (j-l) Glucose uptake, lactate production, and ATP assays were employed to test glucose uptake, lactate production, and ATP accumulation. (m) Extracellular acidification rate (ECAR) was employed to test and detect the glycolytic capacity of DGR cells. (m) , , .