Nerve Growth Factor (NGF) Encourages the Neuroinvasive Potential of Pancreatic Cancer Cells by Activating the Warburg Effect and Promoting Tumor Derived Exosomal miRNA-21 Expression

Figure 8

NGF knockout markedly alleviated nerve invasion by PC cells as well as relieved nociceptive conduction in animal models. (a) The expression of S100 (a neural tissue marker) and CK19 (a cancer cell marker) was detected immunohistochemically (scale bar, 1 mm). (b) The mean left sciatic nerve scores and sciatic nerve index in different groups were measured weekly for 7 weeks. (c) Xenograft tumors and corresponding HE staining of orthotopic sciatic nerves from the mouse hindlimbs. (d-g) Tumor volume, invasive nerve diameter, sciatic nerve score, and sciatic nerve index were tested in each group at 7 weeks after injection. (h) Western blotting was employed to test the protein level of NGF in in mouse tumor tissues. (i) RT-qPCR was employed to test the level of miR-21-5p in mouse tumor tissues. , , .

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