Neonatal Oxidative Stress Impairs Cortical Synapse Formation and GABA Homeostasis in Parvalbumin-Expressing Interneurons

Figure 2

Perineuronal net formation of cortical PVALB+ interneurons. Immunohistochemical staining for parvalbumin (PVALB) and Wisteria floribunda agglutinin (WFA) of 10 μm coronal brain sections obtained from mice after 48 h neonatal hyperoxia (P5-P7) or controls at the ages P14 and P30 (a). The number of PVALB+ interneurons was consistently reduced after exposure to neonatal hyperoxia compared to control (b). At P14, the number of WFA+ cells was not significantly affected by hyperoxia exposure. At P30, formation of WFA+ perineuronal nets was significantly reduced in the cortex of animals previously exposed to hyperoxia compared to control animals (c). In addition, the number of WFA+ and PVALB+ interneurons was reduced at both time points in mice previously exposed to hyperoxia (d). Scale bar 100 μm (P14 and P30 , -test, and ).

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