Dapagliflozin Ameliorates the Formation and Progression of Experimental Abdominal Aortic Aneurysms by Reducing Aortic Inflammation in Mice

Figure 3

Dapagliflozin treatment attenuates aortic leukocyte infiltration. Immunostaining with antibodies against CD68, CD4, CD8, and B220 was used to evaluate the subsets of leukocytes in aneurysmal segment. (a) Immunostaining images of aortic macrophages, CD4⁺ T cells, and CD8⁺ T cells from PPE-infused mice treated with dapagliflozin or vehicle. (b)–(e) Quantification of different subsets of leukocytes per aortic cross-section (ACS) (mean and SD). For normally distributed data, one-way ANOVA analysis was conducted (T cells and B cells analysis), and for data failing normal distribution, the nonparametric Kruskal-Wallis test was performed (macrophage infiltration score). vs. vehicle. DAPA: dapagliflozin; ACS: aortic cross-section. NS: not significant.

(a)

(b)

(c)

(d)

(e)