Osthole Induces Apoptosis and Caspase-3/GSDME-Dependent Pyroptosis via NQO1-Mediated ROS Generation in HeLa Cells

Figure 1

Osthole induces apoptotic and nonapoptotic death in HeLa cells. (a) MTT assay showed that the cell viability was decreased after treatment with osthole at advisable time. (b) Representative bright-field microscopy images (20x, red arrows indicated the large bubbles emerging from the plasma membrane). (c) Representative flow cytometry histograms of Annexin V-PI-stained cells after treatment 18 h in absence or presence of osthole. (d) Percentage (%) of cells stained by Annexin V-FITC and/or PI. (e) LDH release was detected after treatment 18 h in absence or presence of osthole. (f) Representative TEM image (15000x, red arrows indicate autolysosomes; black arrows indicate rupture of plasma membrane). All Data are represented as ; ; , , and compared with the control group.

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