Osthole Induces Apoptosis and Caspase-3/GSDME-Dependent Pyroptosis via NQO1-Mediated ROS Generation in HeLa Cells

Figure 6

NQO1 contributes to ROS overproduction and cell death in HeLa cells. (a) The differences of NQO1 expression were detected by label-free proteomic analysis. (b–e) Incubation with 100 μM DIC for 1 h ahead of treatment with 320 μM osthole. (b) Cell viability was detected by MTT assay. (c) Treatment with osthole for 4 h, DCFH-DA staining was detected by flow cytometry. (d) Western blotting analysis for the expression of apoptosis- and pyroptosis-related proteins. (e) The intensity of bands was quantified by Image J, and β-actin was used as a control. (f–i) NQO1 was knocked down in HeLa cells. (f) Cell viability was detected by MTT assay. (g) DCHF-DA staining was detected by flow cytometry. (h) Western blotting analyzed the expression of apoptosis- and pyroptosis-related proteins. (i) The intensity of bands was quantified by Image J, and β-actin was used as a control. All data are represented as ; ; , , and compared with the control group.

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