Dl-3-n-Butylphthalide Improves Neuroinflammation in Mice with Repeated Cerebral Ischemia-Reperfusion Injury through the Nrf2-Mediated Antioxidant Response and TLR4/MyD88/NF-κB Signaling Pathway

Figure 2

NBP promoted BDNF/TrkB signaling and reduced cell death caused by RCIR injury. (a)–(c) Western blot analysis of BDNF and TrkB in the hippocampus 4 weeks after RCIR. in each group. (d) Representative micrographs for TUNEL staining in CA1 subfields of the hippocampus 4 weeks after RCIR. 400× magnification was shown. . (e) The apoptotic index was calculated as the number of TUNEL (+) cells divided by the total number of cells. in each group. (f)–(h) Western blot analysis demonstrated Bax and Bcl-2 expression at 4 weeks after RCIR injury. in each group. β-Actin was used as an internal control. , the RCIR group vs. the sham group; ^#, ^##, ^###, the NBP80 group or NBP120 group vs. the RCIR group; ^$, the NBP80 group vs. the NBP120 group. Values are expressed as the .

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