Dl-3-n-Butylphthalide Improves Neuroinflammation in Mice with Repeated Cerebral Ischemia-Reperfusion Injury through the Nrf2-Mediated Antioxidant Response and TLR4/MyD88/NF-κB Signaling Pathway

Figure 4

NBP suppressed the activation and regeneration of microglial cells after RCIR. (a) Representative images of double immunofluorescence labeling of Iba-1 (red) and BrdU (green) (200×) in CA1 regions of the hippocampus 4 weeks after RCIR. Iba1⁺/BrdU⁺ cells represented microglial proliferation. . Typical double-labeled areas were magnified. in each group. (b) The number of Iba-1⁺/BrdU⁺ cells in hippocampal CA1 regions. (c, d) Western blot and quantitative analysis of the expressions of Iba-1. in each group. β-Actin was used as an internal control. , the RCIR group vs. sham group; ^#, ^###, the NBP80 group or the NBP120 group vs. the RCIR group; ^$, the NBP80 group vs. NBP120 group. Values are expressed as the . Iba-1: ionized calcium-binding adapter molecule.

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