Dl-3-n-Butylphthalide Improves Neuroinflammation in Mice with Repeated Cerebral Ischemia-Reperfusion Injury through the Nrf2-Mediated Antioxidant Response and TLR4/MyD88/NF-κB Signaling Pathway

Figure 7

NBP significantly suppressed RCIR-induced microglial cell activation and regeneration, but not Nrf2^-/- mice. (a) Representative images of double immunofluorescence labeling of Iba-1 (red) and BrdU (green) (200×) in hippocampal CA1 regions of WT and Nrf2^-/- mice 4 weeks after RCIR. Iba-1⁺/BrdU⁺ cells represented microglial proliferation. Typical double-labeled areas were magnified. . in each group. (b) The number of Iba-1⁺/BrdU⁺ cells in hippocampal CA1 regions. (c, d) Western blot analysis of Iba-1 expressions. in each group. β-Actin was used as an internal control. ^###, the vs. the group; ^&, the group vs. the group; ns: not significant, as indicated. Values are expressed as .

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