MicroRNA-299a-5p Protects against Spinal Cord Injury through Activating AMPK Pathway

Figure 7

The miR-299a-5p agomir activates AMPK through downregulating PHLPP1. (a) The PHLPP1 3-UTR contains a conserved binding site for miR-299a-5p. (b) The luciferase assay indicated that miR-299a-5p directly bound to the PHLPP1 3-UTR. (c)–(e) Quantification of PHLPP1 protein and mRNA levels in SCI mice treated with the agomir, antagomir, or matched NC of miR-299a-5p. (f) The mRNA levels of PHLPP1 in mice with or without PHLPP1 overexpression. (g) Western blot images and quantification of AMPK phosphorylation in the miR-299a-5p agomir-treated mice with or without PHLPP1 overexpression. (h) IL-6 and TNF-α levels in the spinal cord. (i) ROS level detected by DCFH-DA probe. (j) BMS score. (k, l) Sensitivities to mechanical and thermal stimulation. (m) Extravasation of Evans blue dye. for each groups. Data are expressed as the , and was considered statistically significant.

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