Melatonin Alleviates PM_2.5-Induced Hepatic Steatosis and Metabolic-Associated Fatty Liver Disease in ApoE^-/- Mice

Figure 2

Melatonin improved liver oxidative damage induced by PM_2.5. (a) Production of ROS detected by the fluorescent probe DHE (magnification, 200; scale bar, 20 μm). (b) Quantitative analysis of ROS production is reflected by the mean fluorescence intensity as shown in different groups. (c) The level of MDA. (d) The level of 4-HNE. (e) The level of GSH-PX. (f) The vitality of SOD. (g) The mRNA expression of Nrf2, Keap1, and SOD. (h) Western blotting of Nrf2, Keap-1, and SOD. (i) Protein quantification of Nrf2. (j) Protein quantification of Keap1. (k) Protein quantification of SOD. All values are presented as the (). for Con group vs PM_2.5 group and ^# for PM_2.5 group vs PM_2.5+Mel group.

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