Melatonin Alleviates PM_2.5-Induced Hepatic Steatosis and Metabolic-Associated Fatty Liver Disease in ApoE^-/- Mice

Figure 4

PM_2.5 induced lipid accumulation in hepatocytes. (a) Cell viability. (b) Total cholesterol lipid levels (mmol/g). (c) Triacylglycerol lipid levels (mmol/g). (d) Representative fluorescence intensity images obtained from flow cytometry in L02 cells. (e) Analysis of fluorescence intensity obtained from flow cytometry. (f) Representative confocal images of ROS. (g) Western blotting of PTP1B, PP2A, P-PP2A, SP1, P-SP1, and SREBP-1. (h) Protein quantification of PTP1B. (i) Protein quantification of P-PP2A/PP2A. (j) Protein quantification of P-SP1/SP1. (k) Protein quantification of SREBP-1. (l) Protein quantification of Nrf2. (m) Protein quantification of Keap1. (n) Protein quantification of SOD. (o) Western blotting of Nrf2, Keap1, and SOD. All values are presented as the . .

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