Research Article
Melatonin Alleviates PM2.5-Induced Hepatic Steatosis and Metabolic-Associated Fatty Liver Disease in ApoE-/- Mice
Figure 6
Melatonin alleviated PM2.5-induced oxidative damage and upregulated PTP1B expression in vitro. (a) and (b) Cell ability. (c) Total cholesterol lipid levels (mmol/g). (d) Triacylglycerol lipid levels (mmol/g). (e) Representative confocal images of ROS. (f) Representative fluorescence intensity images obtained from flow cytometry in L02 cells. (g) Analysis of fluorescence intensity obtained from flow cytometry. (h) The mRNA expression of PTP1B, PP2A, SP1, and SREBP-1. (i) Western blotting of PTP1B, PP2A, P-PP2A, SP1, P-SP1, and SREBP-1. (j) Protein quantification of PTP1B. (k) Protein quantification of P-PP2A/PP2A. (l) Protein quantification of P-SP1/SP1. (m) Protein quantification of SREBP-1. (n) The activity of PP2A. (o) The activity of SP1. (p) The mRNA expression of Nrf2, Keap-1, and SOD. (q) Western blotting of Nrf2, Keap-1, and SOD. (r) Protein quantification of Nrf2. (s) Protein quantification of Keap-1. (t) Protein quantification of SOD. All values are presented as the . for Con group vs PM2.5 group and # for PM2.5 group vs PM2.5+Mel group.
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