Inhibition of PFKFB Preserves Intestinal Barrier Function in Sepsis by Inhibiting NLRP3/GSDMD

Figure 6

3PO protects against pyroptosis in vitro and in vivo. (a, b) Pyroptosis-related proteins, including NLRP3, cleaved caspase-1, IL-1β, and IL-18, were examined by Western blot in intestinal tissue of mice. (c, d) The expression of GSDMD was detected by Western blot in intestinal tissue of mice. si-Gsdmd was used to inhibit pyroptosis and reveal the relationship between 3PO and pyroptosis. (e) Levels of the released LDH were detected in C2C12 culture medium supernatant. (f) Cell viability was determined using the CCK-8 assay. The values were shown as (). Statistical analysis was performed using a -test (b, d–f). compared with the no-treatment group and ^### compared with the CLP treatment group. ^&& compared with the si-Con group and ^$$ compared with the LPS+si-Con group. 3PO protected against CLP-induced intestinal injury by suppressing NLRP3/caspase-1/GSDMD.

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