miR-409-3p Regulated by GATA2 Promotes Cardiac Fibrosis through Targeting Gpd1

Figure 2

Deficiency of miR-409-3p reverses MI-induced cardiac fibrosis and cardiac dysfunction. C57BL/6 mice were injected with miR-409-3p antagomir or nc antagomir via tail vein for three consecutive days and subjected to sham or MI surgery for 3 weeks. (a) The expression of miR-409-3p in heart tissues measured by qRT-PCR. (b) Masson’s trichrome staining and quantification of collagen deposition in heart sections. (c) Cardiac function recorded by echocardiography. (d) Proliferation of cardiac fibroblasts determined by immunofluorescent staining of Ki67 and Vimentin. (e) Fibroblast-to-myofibroblast differentiation determined by immunofluorescent staining of α-SMA and Vimentin. (f) Expression of fibrotic markers (α-SMA, Col1a1, and Col3a1) measured by qRT-PCR. (g) Protein levels of CTGF, α-SMA, Col1a1, and Col3a1 in heart tissues (). Scale bar: 100 μm. , , and versus respective controls.

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