miR-409-3p Regulated by GATA2 Promotes Cardiac Fibrosis through Targeting Gpd1

Figure 3

miR-409-3p mimic promotes cardiac fibrosis in vitro. (a) Expression of miR-409-3p in cultured NRCFs transfected with miR-409-3p mimic (). (b, c) mRNA and protein levels of fibrosis-related genes in NRCFs transfected with miR-409-3p mimic and subjected to Ang II stimulation ( for qRT-PCR, for western blot). (d) Proliferation and differentiation degree of NRCFs determined by immunofluorescent staining of EdU and α-SMA (). (e, f) Expression of fibrotic genes in NRCFs transfected with miR-409-3p mimic in the presence or absence of TGF-β treatment ( for qRT-PCR, for western blot). (g) Proliferation and differentiation degree of NRCFs evidenced with EdU/α-SMA staining (). Scale bar: 200 μm. , , and versus respective controls.

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