Research Article
miR-409-3p Regulated by GATA2 Promotes Cardiac Fibrosis through Targeting Gpd1
Figure 4
Inhibition of miR-409-3p suppresses cardiac fibrosis in NRCF. (a) miR-409-3p level in NRCFs transfected with miR-409-3p inhibitor measured by qRT-PCR (). (b, c) qRT-PCR and western blot were, respectively, conducted to detect levels of fibrosis-related genes in NRCFs transfected with miR-409-3p inhibitor and subjected to Ang II stimulation ( for qRT-PCR, for western blot). (d) Proliferation and differentiation degree of NRCFs determined by immunofluorescent staining of EdU and α-SMA (). (e, f) Expression of fibrotic genes in NRCFs transfected with miR-409-3p inhibition in the presence or absence of TGF-β treatment ( for qRT-PCR, for western blot). (g) NRCF proliferation and differentiation into myofibroblasts evidenced by EdU/α-SMA staining (). Scale bar: 200 μm. , , and versus respective controls.
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