miR-409-3p Regulated by GATA2 Promotes Cardiac Fibrosis through Targeting Gpd1

Figure 5

miR-409-3p affects cardiac fibrosis by targeting Gpd1. (a) Three prediction databases were used to search for the potential target gene of miR-409-3p. (b) Dysregulated genes in heart tissues of post-MI mice versus sham mice (). (c) Direct target of miR-409-3p identified by luciferase reporter assays (). (d) Protein levels of Gpd1 in NRCFs treated with miR-409-3p mimic or inhibitor (). (e) Expression of Gpd1 in heart tissues from post-MI mice versus sham mice detected by western blot (). (f) Protein levels of Gpd1 in two cell models of cardiac fibrosis induced by Ang II or TGF-β (). (g) Silence efficiency of Gpd1 siRNA detected by qRT-PCR and western blot (). (h–j) The effect of Gpd1 knockdown in NRCFs transfected with miR-409-3p inhibitor on fibrotic gene expression, cell proliferation, and differentiation into myofibroblasts ( for qRT-PCR and immunofluorescent staining, for western blot). Scale bar: 200 μm. , , and versus respective controls.

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