miR-409-3p Regulated by GATA2 Promotes Cardiac Fibrosis through Targeting Gpd1

Figure 6

GATA2 is a fibrosis-associated positive regulatory transcription factor of miR-409-3p. (a) Transcription factors of miR-409-3p were searched in the TransmiR database. (b, c) miR-409-3p expression in NRCFs transfected with GATA2 overexpression plasmid or siRNA (). (d) Expression of GATA2 in heart tissues from post-MI mice compared to sham mice (). (e) Protein levels of GATA2 in NRCFs treated with Ang II or TGF-β (). (f–h) The effect of Gpd1 knockdown in NRCFs overexpressing GATA2 on fibrotic gene expression, cell proliferation, and differentiation into myofibroblasts ( for qRT-PCR and immunofluorescent staining, for western blot). Scale bar: 200 μm. , , and versus respective controls.

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