Theaflavin-3,3-Digallate Plays a ROS-Mediated Dual Role in Ferroptosis and Apoptosis via the MAPK Pathway in Human Osteosarcoma Cell Lines and Xenografts

Figure 4

TF3 inhibited proliferation in OS cell lines. OS cells were treated with 0.05% DMSO (vehicle control) or the indicated concentrations (0, 40, 80, 120, or 160 μM) of TF3 for the indicated times. (a) The colony formation ability of MG-63 and HOS cells was determined. (b) Quantitative analyses of the colonies in (a). (c, d) The proliferation of MG-63 and HOS cells was assessed through EdU staining and quantification. (e) Western blotting and statistical analyses of proliferation-related proteins, including Ki67, PCNA, and P21, in MG-63 and HOS cells. The data are presented as the (). , , , and vs. the vehicle control group.

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