Theaflavin-3,3-Digallate Plays a ROS-Mediated Dual Role in Ferroptosis and Apoptosis via the MAPK Pathway in Human Osteosarcoma Cell Lines and Xenografts

Figure 8

TF3 induced cell death in OS cell lines via ROS accumulation. (a) Colony formation assay of MG63 and HOS cells treated with 160 μM TF3 with or without 2 mM NAC for 24 h and statistical analysis of the colony formation assay results. (b) Flow cytometry of MG63 and HOS cells treated with 160 μM TF3 with or without 2 mM NAC for 24 h and quantitative analysis of the flow cytometry results. (c) Western blotting and statistical analyses of MAPK signalling pathway-related proteins, including p-JNK, p-P38, p-ERK, JNK, P38, and ERK, in MG63 and HOS cells treated with 160 μM TF3 with or without 2 mM NAC for 24 h. The data are presented as the (). , , , and vs. the vehicle control group.

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