Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying circ_0050205 Attenuate Intervertebral Disc Degeneration

Figure 1

BMSC-EVs enhance the survival of NPCs and inhibit ECM degradation in NPCs. (a) Characterization of BMSC-EVs by TEM observation (100 nm). (b) NanoSight analysis of the size of BMSC-EVs in diameter. (c) Western blot of EV-specific surface markers CD63, calnexin, and TSG101 in BMSC-EVs, vs. the EV group. (d) NPC uptake of PKH67-labeled BMSC-EVs by NPCs (25 μm). (e) CCK-8 assay for viability of 50 μM tBHP-exposed NPCs for 12 h following coculture with BMSC-EVs. (f) EdU staining for proliferation of 50 μM tBHP-exposed NPCs for 12 h following coculture with BMSC-EVs. (g) Flow cytometric analysis of apoptosis of 50 μM tBHP-exposed NPCs for 12 h following coculture with BMSC-EVs. (h) Immunofluorescence detection of MMP13, ADAMTS 5, COL II, and aggrecan proteins in 50 μM tBHP-exposed NPCs for 12 h following coculture with BMSC-EVs. (i) The ROS production was examined using DCF-DA kit in 50 μM tBHP-exposed NPCs for 12 h following coculture with BMSC-EVs (25 μm). (j) Lipid peroxidation determined by C11-BODIPY581/591 in 50 μM tBHP-exposed NPCs for 12 h following coculture with BMSC-EVs. (k) The total free iron in 50 μM tBHP-exposed NPCs for 12 h following coculture with BMSC-EVs. vs. the tBHP group. Cell experiments were conducted three times independently.

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