Research Article
Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying circ_0050205 Attenuate Intervertebral Disc Degeneration
Figure 3
circ_0050205 functions as a ceRNA to sponge miR-665. (a) Venn diagram showing common downstream miRNAs of circ_0050205 in the Circbank and CircInteractome databases. (b) The binding sites between circ_0050205 and miR-665. (c) miR-665 expression in mouse NP tissues of IDD mice () and sham-operated mice () determined by RT-qPCR, vs. sham-operated mice. (d) miR-665 expression in tBHP-exposed NPCs determined by RT-qPCR, vs. tBHP 0 μM. (e) circ_0050205 binding with miR-665 was examined by luciferase activity assay, vs. mimic NC. (f) Enrichment of circ_0050205 and miR-665 in the immunoprecipitates using anti-Ago2 or IgG, vs. IgG. (g) RNA pulldown demonstrated that more miR-665 was pulled down using Bio-circ_0050205-WT and Bio-circ_0050205-Mut, vs. Bio-miR-665-Mut. (h) circ_0050205 overexpression or knockdown efficiency in NPCs determined by RT-qPCR, vs. vector, vs. sh-NC. (i) RT-qPCR analysis of circ_0050205 and miR-665 expression in tBHP-exposed NPCs treated with LV-circ_0050205 or sh-circ_0050205. vs. tBHP + vector or tBHP + sh-NC. Cell experiments were conducted three times independently.
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