Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying circ_0050205 Attenuate Intervertebral Disc Degeneration

Figure 4

circ_0050205upregulates GPX4 expression by acting as a asmiR-665 sponge. (a) GPX4expression in mouse NP tissues of IDD mice () and sham-operated mice () determined by RT-qPCR, vs. sham-operated mice. (b) GPX4 expression in tBHP-exposed NPCs determined by RT-qPCR, vs. tBHP 0 μM. (c) Pearson correlation analysis of miR-665 expression and GPX4 expression in the NP tissues of IDD mice (). (d) miR-665 binding to GPX4 examined by luciferase activity assay, vs. mimic NC. (e) RNA pulldown demonstrated that Bio-miR-665-WT bound more GPX4 than Bio-miR-665-Mut, vs. Bio-miR-665-MUT. (f) miR-665 expression in NPCs following miR-665 mimic and inhibitor transfection determined by RT-qPCR. vs. mimic NC, vs. inhibitor NC. (g) RT-qPCR analysis of GPX4 expression in tBHP-exposed NPCs after miR-665 overexpression or inhibition, vs. tBHP + mimic NC or tBHP + inhibitor NC. (h) RT-qPCR analysis of GPX4 expression in tBHP-exposed NPCs treated with LV-circ_0050205 or combined with miR-665 mimic. vs. tBHP + vector, vs. tBHP + LV-circ_0050205 + mimic NC. Cell experiments were conducted three times independently.

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