Research Article
Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying circ_0050205 Attenuate Intervertebral Disc Degeneration
Figure 5
GPX4 contributes to the survival of NPCs and reduces ECM degradation in NPCs. (a) GPX4 overexpression efficiency in NPCs confirmed by RT-qPCR. (b) CCK-8 assay of viability of tBHP-exposed NPCs treated with LV-GPX4. (c) EdU staining of proliferation of tBHP-exposed NPCs treated with LV-GPX4. (d) Flow cytometric analysis of apoptosis of tBHP-exposed NPCs treated with LV-GPX4. (e) Immunofluorescence detection of MMP13, ADAMTS 5, COL II, and aggrecan proteins in tBHP-exposed NPCs treated with LV-GPX4 (25 μm). (f) The ROS production was examined using the DCF-DA kit in tBHP-exposed NPCs treated with LV-GPX4. (g) Lipid peroxidation determined by C11-BODIPY581/591 in tBHP-exposed NPCs treated with LV-GPX4. (h) The total free iron in tBHP-exposed NPCs treated with LV-GPX4. vs. tBHP + vector. Cell experiments were conducted three times independently.
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