Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying circ_0050205 Attenuate Intervertebral Disc Degeneration

Figure 7

circ_0050205 in BMSC-EVs attenuates IDD progression by regulating the miR-665/GPX4 axis. A total of 40 C57BL/6J male mice were randomly assigned into the sham group, IDD group, BMSC-EV + vector group, BMSC-EV + LV-circ_0050205 + sh-NC group, and BMSC-EV + LV-circ_0050205 + sh-GPX4 group, with 8 mice per group. (a) X-ray examination of degenerative characteristics of IDD mice and DHI (%). (b) Histological examinations of mouse NP tissues by HE staining and oil red O staining (50 μm). (c) Histological scores. (d) Immunohistochemical staining of COL II, aggrecan, and GPX4 proteins in mouse NP tissues. (e) Immunohistochemical staining of MMP13 and ADAMTS 5 proteins in mouse NP tissues. (f) The total free iron in mouse NP tissues. (g) RT-qPCR analysis of circ_0050205, miR-665, and GPX4 expression in mouse NP tissues. vs. sham, vs. IDD, vs. BMSC-EVs + vector, vs. BMSC-EVs + LV-circ_0050205 + sh-NC.

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