lncRNA ZFAS1 Positively Facilitates Endothelial Ferroptosis via miR-7-5p/ACSL4 Axis in Diabetic Retinopathy

Figure 2

Inhibition of ZFAS1 rescued HG-induced ferroptosis in hRECs. (a) Both apoptosis inhibitor TUDCA and ferroptosis inhibitor Fer-1 administration rescued the downregulated cell viability in HG group. Compared to HG control, there is no difference in total (b) or ferrous iron level (c), lipid hydroperoxide accumulation (d and e), nor GPx4 expression level (f) after 10 μM apoptosis inhibitor TUDCA, necrosis inhibitor necrostatin-1, pyroptosis inhibitor TETD treatment for 48 h, while transfection of Sh-ZFAS1 remarkably lightened the above ferroptosis phenotypes. replicates. . 10 μM Fer-1 treatment for 48 h notably ameliorated the HG-induced ferroptosis-related phenotypes indicated above. replicates. Data are presented as ± SEM. Ns: no significant; and . Scale bar, 50 μm.

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