Tetrahydrofolate Alleviates the Inhibitory Effect of Oxidative Stress on Neural Stem Cell Proliferation through PTEN/Akt/mTOR Pathway

Figure 1

NSC culture and characteristics. (a) Morphological changes of cultured NSC under phase-contrast microscope from 6 to 48 hours in floating condition. Scale bar: 50 μm. (b) Immunostaining images represented that the majority of cultured cells were positive for Nestin and/or Sox2. Scale bar: 20 μm. (c) Immunostaining images showed that cultured cells could differentiate into GFAP⁺ cells after 7 days in differentiation medium. Scale bars: 10 μm. (d) Immunostaining pictures indicated that cultured cells held the potential of differentiation into MAP2⁺ cells after 7 days in differentiation medium. Scale bars: 10 μm. (e) Immunostaining images presented cultured cells possessed the capacity of transformation into Olig2⁺ cells after 7 days in differentiation medium. Scale bars: 10 μm. (f) Histogram summarizing the percentage of cultured cell differentiation into GFAP⁺, MAP2⁺, and Olig2⁺ cells, respectively.

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