Tetrahydrofolate Alleviates the Inhibitory Effect of Oxidative Stress on Neural Stem Cell Proliferation through PTEN/Akt/mTOR Pathway

Figure 3

THF reversed the inhibitory effect of oxidative stress on NSC proliferation. (a) The absorbance value in each group at 24 h and 48 h using CCK-8 assays. , vs. control group; ^# vs. H₂O₂ group. (b) Growth curve analysis in different groups. , , vs. control group; ^#, ^## vs. H₂O₂ group. (c) The phase-contrasted images depicting NSC condition in each group at different timepoints. Scale bars: 50 μm. (d) Bar graph summarizing the number of neurospheres per field among different groups at 24 h. , vs. control group; ^# vs. H₂O₂ group. (e) Bar chart representing the number of neurospheres per field among different groups at 48 h. , vs. control group; ^# vs. H₂O₂ group. (f) Histogram illustrating the average diameter of neurospheres in each group at 24 h. (g) Qualification of the average diameter of neurospheres in each group at 48 h. , vs. control group; ^### vs. H₂O₂ group. (h) The immunostaining of Nestin and Ki67 in various groups. Scale bars: 10 μm. (i) The statistical analysis of Nestin⁺Ki67⁺ from (h). , vs. control group; ^# vs. H₂O₂ group.

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