Tetrahydrofolate Alleviates the Inhibitory Effect of Oxidative Stress on Neural Stem Cell Proliferation through PTEN/Akt/mTOR Pathway

Figure 6

PTEN KD played the same role as THF in reducing oxidative stress in NSC. (a) Mitochondrial ROS level in different groups. Scale bar: 10 μm. (b) Bar graph illustrating the optic intensity from (a). , vs. scramble group; ^### vs. scramble + H₂O₂ group. (c) Bands demonstrating the expression of SOD1 and SOD2 in each group. β-Actin was used as a loading control. (d) Semiquantitation of SOD1 from (c). , vs. scramble group; ^# vs. scramble + H₂O₂ group. (e) Semiquantification of SOD2 from (c). , vs. scramble group; ^#,^## vs. scramble + H₂O₂ group.

(a)

(b)

(c)

(d)

(e)