Tetrahydrofolate Alleviates the Inhibitory Effect of Oxidative Stress on Neural Stem Cell Proliferation through PTEN/Akt/mTOR Pathway

Figure 8

PTEN KD exerted the same effect as THF on retrieving NSC proliferation potential suppressed by oxidative stress. (a) The phase-contrasted images depicting NSC growth in each group at different timepoints. Scale bars: 50 μm. (b) Bar graph summarizing the number of neurospheres per field among different groups at 24 h. , vs. scramble group; ^#, ^## vs. scramble + H₂O₂ group. (c) Bar chart demonstrating the number of neurospheres per field among different groups at 48 h. , vs. scramble group; ^#, ^## vs. scramble + H₂O₂ group. (d) Histogram illustrating the average diameter of neurospheres in each group at 24 h. , vs. scramble group. (e) Qualification of the average diameter of neurospheres in each group at 48 h. , vs. scramble group; ^#, ^## vs. scramble + H₂O₂ group. (f) The immunostaining of Nestin and Ki67 in various groups. Scale bars: 50 μm.

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