HBO Alleviates Neural Stem Cell Pyroptosis via lncRNA-H19/miR-423-5p/NLRP3 Axis and Improves Neurogenesis after Oxygen Glucose Deprivation

Figure 1

HBO inhibited the activation of NLRP3/Caspase-1/GSDMD signaling in NSCs following OGD. (a, b) NSC viability and release of LDH were tested following OGD exposure and HBO treatment in diverse pressure. (c) The protein expression of NLRP3, Caspase-1 p20, and GSDMD-N in Con, OGD, OGD+HBO, and OGD+HBO+Nig groups was determined by WB. (d–f) Quantitative analysis on the expression of NLRP3, Caspase-1 p20, and GSDMD-N protein in the four groups. (g, h) The level of IL-1β and IL-18 produced by NSCs in the four groups was measured by ELISA. vs. the Con group. ^# vs. the OGD group. ^† vs. the OGD+HBO group.

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