Downregulation of SIRT3 Aggravates Lung Ischemia Reperfusion Injury by Increasing Mitochondrial Fission and Oxidative Stress through HIF-1<i>α</i>-Dependent Mechanisms

<div>Expression of SIRT3 increased after lung I/R <i>in vivo</i>. Mice were treated with 3-TYP (50 mg/kg, i.p.) every 2 days for three times before surgery. (a) SIRT3 protein levels were detected by western blotting. (b) Protein expression of SIRT3 relative to <i>β</i>-actin. (c) Levels of SIRT3 mRNA. (d) Immunofluorescence micrographs of SIRT3 (red) and F4/80 (green) from mice of the different treatment groups. DAPI was used to stain nuclei. Scale bar: 200 <i>μ</i>m. 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Oxidative Medicine and Cellular Longevity

fig1

Figure 1

Figure 1: Downregulation of SIRT3 Aggravates Lung Ischemia Reperfusion Injury by Increasing Mitochondrial Fission and Oxidative Stress through HIF-1<i>α</i>-Dependent Mechanisms 

Figure 1 | Downregulation of SIRT3 Aggravates Lung Ischemia Reperfusion Injury by Increasing Mitochondrial Fission and Oxidative Stress through HIF-1α-Dependent Mechanisms 