Downregulation of SIRT3 Aggravates Lung Ischemia Reperfusion Injury by Increasing Mitochondrial Fission and Oxidative Stress through HIF-1α-Dependent Mechanisms

Figure 2

Inhibition of SIRT3 aggravated lung injury and inflammation after lung I/R in vivo. Histopathological injury, W/D ratios, ultrastructural changes, and inflammatory response were determined 24 h after reperfusion. (a) H&E staining of mouse lung tissue ( per group). Scale bar: 200 μm and 100 μm. (b) Pathological scores were assessed based on images in panel (a). (c) W/D ratios of lung tissues. (d) Levels of IL-6 and (e) IL-1β in lung tissue homogenate as measured by ELISA. (f) Levels of IL-6 and (g) IL-1β in BALF as measured by ELISA. The data are expressed as ( for panels b, c, d, e, f, g), *. (h) Ultrastructural changes were assessed using TEM ( per group). Arrows indicate lamellar bodies. Scale bar, 2 μm.

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