Downregulation of SIRT3 Aggravates Lung Ischemia Reperfusion Injury by Increasing Mitochondrial Fission and Oxidative Stress through HIF-1α-Dependent Mechanisms

Figure 4

Inhibition of SIRT3 decreased the number of mitochondria, increased the levels of Drp-1 and Fis-1, and led to excessive mitochondrial fission after I/R in vivo. (a) Mitochondrial changes were assessed using TEM. Scale bar, 2 μm. (b) Drp-1, Fis-1, and Mfn-2 protein levels detected by western blotting. (c–e) Drp-1, Fis-1, and Mfn-2 protein expression relative to β-actin. (f–h) Lung Drp-1 and Fis-1 protein expression detected by immunohistochemistry and semiquantitative analysis of expression in representative lung sections. Scale bar: 200 μm and 100 μm. The data are expressed as ( for all panels), *.

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