Downregulation of SIRT3 Aggravates Lung Ischemia Reperfusion Injury by Increasing Mitochondrial Fission and Oxidative Stress through HIF-1α-Dependent Mechanisms

Figure 5

Inhibition of SIRT3 increased the expression of HIF-1α after lung I/R in vivo. (a) HIF-1α protein levels by western blotting. (b) Expression of HIF-1α protein relative to β-actin. (c) Levels of HIF-1α mRNA. (d) Immunofluorescence micrographs of HIF-1α (red) and F4/80 (green) in lung tissues. DAPI was used to stain nuclei. Scale bar: 200 μm. The data are expressed as ( for all panels), *.

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