Downregulation of SIRT3 Aggravates Lung Ischemia Reperfusion Injury by Increasing Mitochondrial Fission and Oxidative Stress through HIF-1α-Dependent Mechanisms

Figure 8

Inhibition of Drp-1 reduced mitochondrial fission, decreased mitochondrial oxidative stress, increased ATP content and MMP after H/R in vitro. RAW264.7 cells were subjected to Mdivi-1 treatment (10 μm) for 1 h before induction of hypoxia. (a) Cell viability of RAW264.7 cells after treatment with different concentrations of Mdivi-1 measured with the CCK-8 kit. (b) Western blotting of Drp-1 protein. (c) Expression of Drp-1 protein relative to β-actin. (d–f) MDA, SOD₂, and GSH levels in the supernatant of RAW264.7 cells. (g) The content of ATP. (h, i) MitoSOX fluorescence staining and fluorescence intensity. (j) Representative images showing the change in MMP. The data are expressed as ( for all panels), *.

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