Activation of SIRT1 Alleviates Ferroptosis in the Early Brain Injury after Subarachnoid Hemorrhage

Figure 2

Ferroptosis-related pathways activated in the temporal cortex after SAH. (a) Western blotting assay for ACSL4 expression in the temporal cortex after SAH. (b) Quantification of ACSL4 expression. (c) Representative images of IF staining for ACSL4. (d) Western blotting assay for TFR, DMT1, ferritin, and FPN expression in the temporal cortex after SAH. (e–h) Quantification of the expression of TFR, DMT1, ferritin, and FPN. (i) Representative images of IF staining for FTH. (j) Quantification of ferrous iron content. (k) Western blotting assay for XCT and GPX4 expression. (l, m) Quantification of the expression of XCT and GPX4. (n) Representative images of IF staining for GPX4. (o) Western blotting assay for FSP1 and CoQ10B expression. (p, q) Quantification of the expression of FSP1 and CoQ10B. /group. Bars represent the . , , and . . ASCL4: acyl-CoA synthetase long-chain family member 4; TFR: transferrin receptor; DMT1: divalent metal transporter 1; FPN: ferroportin; FTH: ferritin heavy chain; GPX4: glutathione peroxidase 4; FSP1: ferroptosis suppressor protein 1.

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