Activation of SIRT1 Alleviates Ferroptosis in the Early Brain Injury after Subarachnoid Hemorrhage

Figure 3

Ferroptosis-related pathways activated in HT-22 cells exposed to oxyHb. (a) Representative images of TEM. (b) Western blotting assay for ACSL4 expression in all groups. (c) Quantification of the expression of ACSL4. (d) Representative images of IF staining for ACSL4. (e) Western blotting assay for TFR, DMT1, ferritin, and FPN expression. (f–i) Quantification of TFR, DMT1, ferritin, and FPN expression. (j) Representative images of ferrous iron staining. (k) Representative images of IF staining for FTH. (l) Western blotting assay for XCT and GPX4 expression in all groups. (m, n) Quantification of XCT and GPX4 expression. (o) Representative images of IF staining for GPX4. (p) Western blotting assay for FSP1 and CoQ10B expression in all groups. (q, r) Quantification of the expression of FSP1 and CoQ10B. (s) Quantitative analysis of MDA. /group. Bars represent the . , , , and . . TEM: transmission electron microscope; ASCL4: acyl-CoA synthetase long-chain family member 4; TFR: transferrin receptor; DMT1: divalent metal transporter 1; FPN: ferroportin; FTH: ferritin heavy chain; GPX4: glutathione peroxidase 4; FSP1: ferroptosis suppressor protein 1; MDA: malondialdehyde.

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