Activation of SIRT1 Alleviates Ferroptosis in the Early Brain Injury after Subarachnoid Hemorrhage

Figure 4

Inhibition of ferroptosis by Fer-1 alleviated oxidative stress-induced brain injury via upregulating the expression of GPX4. (a) Western blotting assay for XCT, GPX4, CoQ10B, and FSP1 expression in different groups in vivo. (b–e) Quantification of the expression of XCT, GPX4, CoQ10B, and FSP1. (f) Quantitative analysis of MDA in different groups in vivo. (g) Western blotting assay for the expression of GPX4 in all groups with different doses. (h) Quantification of GPX4 expression. (i) Western blotting assay for the expression of XCT, GPX4, CoQ10B, and FSP1 in different groups in vitro. (j–m) Quantification of the expression of XCT, GPX4, CoQ10B, and FSP1. (n) Quantitative analysis of MDA. /group. Bars represent the . and . GPX4: glutathione peroxidase 4; FSP1: ferroptosis suppressor protein 1; MDA: malondialdehyde.

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