Activation of SIRT1 Alleviates Ferroptosis in the Early Brain Injury after Subarachnoid Hemorrhage

Figure 5

SRIT1 activation by RSV inhibited ferroptosis following SAH in vivo and in vitro. (a) Western blotting assay for SIRT1 expression in the temporal cortex after SAH. (b) Quantification of SIRT1 expression. (c) Representative images of IF staining for SIRT1. (d) Western blotting assay for SIRT1, XCT, GPX4, CoQ10B, and FSP1 expression in different groups in vivo. (e–i) Quantification of SIRT1, XCT, GPX4, CoQ10B, and FSP1 expression. (j) Quantitative analysis of MDA in different groups in vivo. (k) Western blotting assay for SIRT1 expression in all groups in vitro. (l) Quantification of the expression of SIRT1. (m) Western blotting assay for SIRT1 and GPX4 expression in all groups with different doses. (n, o) Quantification of the expression of SIRT1 and GPX4. (p) Western blotting assay for SIRT1, XCT, GPX4, CoQ10B, and FSP1 expression in different groups in vitro. (q–u) Quantification of the expression of SIRT1, XCT, GPX4, CoQ10B, and FSP1. (v) Quantitative analysis of MDA. /group. Bars represent the . . and . RSV: resveratrol; GPX4: glutathione peroxidase 4; FSP1: ferroptosis suppressor protein 1; MDA: malondialdehyde.

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